Research in Drug Development (RD3)

Pharmacognosy Bioanalysis and Drug Discovery

Contact
 

Research activities

His research field is the analysis of posttranslational modifications (PTMs) of proteins by mass spectrometry. The targeted PTMs are the oxidative ones (oxidation of tryptophan, tyrosine, lysine, cysteine, …) in the context of chronic inflammation pathologies/oxidative stress like in atherosclerosis/cardiovascular disease. Another type of PTMs that he studies, is the glycosylation. Glycosylation is the most abundant modification of proteins, and in the context of therapeutic proteins, more than 60 % of the currently approved biotherapeutics are glycosylated. His research is also devoted to the development of analytical tools for the other “omics” fields mainly metabolomics and proteomics. He currently develops his research projects in the RD3-PBM unit and the Analytical Platform of the Faculty of Pharmacy

Top ten recent articles

Targeted and Untargeted Mass Spectrometry-Based Metabolomics for Chemical Profiling of Three Coffee Species

Montis, A., Souard, F., Delporte, C., Stoffelen, P., Stevigny, C., & Van Antwerpen, P. (2022). Targeted and Untargeted Mass Spectrometry-Based Metabolomics for Chemical Profiling of Three Coffee Species. Molecules (Print Archive Edition), 27(10), 3152. doi:10.3390/molecules27103152  

 

Untargeted metabolomics approach to discriminate mistletoe commercial products

Vanhaverbeke, C., Touboul, D., Elie, N., Prévost, M., Meunier, C. C., Michelland, S. S., Cunin, V. V., Ma, L., Vermijlen, D., Delporte, C., Pochet, S., Le Gouellec, A. A., Sève, M. M., Van Antwerpen, P., & Souard, F. (2021). Untargeted metabolomics approach to discriminate mistletoe commercial products. Scientific report, 11, 10.1038/s41598-021-93255-z, 14205.  

 

Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS.

Helali, Y., Sharma, S., Vandeput, M., Welba, D., Van Antwerpen, P., Marchant, A., & Delporte, C. (2021). Fc Glycosylation Characterization of Human Immunoglobulins G Using Immunocapture and LC-MS. Methods in molecular biology, 2271, 57-71. doi:10.1007/978-1-0716-1241-5_4  

 

FTIR spectroscopy as an analytical tool to compare glycosylation in therapeutic monoclonal antibodies

Derenne, A., Derfoufi, K.-M., Cowper, B., Delporte, C., & Goormaghtigh, E. (2020). FTIR spectroscopy as an analytical tool to compare glycosylation in therapeutic monoclonal antibodies. Analytica chimica acta, 1112, 62-71. doi:10.1016/j.aca.2020.03.038  

 

The other myeloperoxidase: Emerging functions

Vanhamme, L., Zouaoui Boudjeltia, K., Van Antwerpen, P., & Delporte, C. (2018). The other myeloperoxidase: Emerging functions. Archives of biochemistry and biophysics, 649, 1-14. doi:10.1016/j.abb.2018.03.037  

 

Native and myeloperoxidase-oxidized low-density lipoproteins act in synergy to induce release of resolvin-D1 from endothelial cells.

Dufour, D., Khalil, A., Nuyens, V., Rousseau, A., Delporte, C., Noyon, C., Cortese, M., Reye, F., Pireaux, V., Neve, J., Vanhamme, L., Robaye, B., Lelubre, C., Desmet, J.-M., Raes, M., Zouaoui Boudjeltia, K., & Van Antwerpen, P. (2018). Native and myeloperoxidase-oxidized low-density lipoproteins act in synergy to induce release of resolvin-D1 from endothelial cells. Atherosclerosis, 272, 108-117. doi:10.1016/j.atherosclerosis.2018.03.012  

 

Metabolomics fingerprint of coffee species determined by untargeted-profiling study using LC-HRMS

Souard, F., Delporte, C., Stoffelen, P., Thévenot, E. E., Noret, N., Dauvergne, B., Kauffmann, J.-M., Van Antwerpen, P., & Stevigny, C. (2018). Metabolomics fingerprint of coffee species determined by untargeted-profiling study using LC-HRMS. Food chemistry, 245, 603-612. doi:10.1016/j.foodchem.2017.10.022  

 

Differential Effects of E-Cigarette on Microvascular Endothelial Function, Arterial Stiffness and Oxidative Stress: A Randomized Crossover Trial

Chaumont, M., De Becker, B., Zaher, W., Culié, A., Deprez, G., Melot, C., Reye, F., Van Antwerpen, P., Delporte, C., Debbas, N., Zouaoui Boudjeltia, K., & Van De Borne, P. (2018). Differential Effects of E-Cigarette on Microvascular Endothelial Function, Arterial Stiffness and Oxidative Stress: A Randomized Crossover Trial. Scientific reports, 8(1), 10378. doi:10.1038/s41598-018-28723-0  

 

Batch-to-batch N-glycosylation Study of Infliximab, Trastuzumab, and Bevacizumab, and Stability Study of Bevacizumab

Planinc, A., Dejaegher, B., Vander Heyden, Y., Viaene, J., Van Praet, S., Rappez, F., Van Antwerpen, P., & Delporte, C. (2017). Batch-to-batch N-glycosylation Study of Infliximab, Trastuzumab, and Bevacizumab, and Stability Study of Bevacizumab. European journal of hospital pharmacy, 24(5), 286-292. doi:10.1136/ejhpharm-2016-001022  

 

LC-MS analysis combined with principal component analysis and soft independent modelling by class analogy for a better detection of changes in N-glycosylation profiles of therapeutic glycoproteins.

Planinc, A., Dejaegher, B., Vander Heyden, Y., Viaene, J., Van Praet, S., Rappez, F., Van Antwerpen, P., & Delporte, C. (2017). LC-MS analysis combined with principal component analysis and soft independent modelling by class analogy for a better detection of changes in N-glycosylation profiles of therapeutic glycoproteins. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-016-9683-9